TIM3 Polyklonaler Antikörper

TIM3 Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 11872-1-AP

Synonyme

CD366, HAVcr 2, HAVCR2, KIM 3, T cell membrane protein 3, Tim 3, TIM3, TIM-3, TIMD 3, TIMD3

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Galerie der Validierungsdaten

• 

  WB analysis using 11872-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11872-1-AP (TIM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 11872-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 11872-1-AP (TIM3 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 11872-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11872-1-AP (TIM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  FC experiment of Raji using 11872-1-AP

  1X10^6 Raji cells were stained with 0.20ug TIM3 antibody (11872-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, Raji-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in FC: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

11872-1-AP bindet in WB, IHC, FC, ELISA TIM3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TIM3 fusion protein Ag2459
• Vollständiger Name: hepatitis A virus cellular receptor 2
• Berechnetes Molekulargewicht: 301 aa, 33 kDa
• Beobachtetes Molekulargewicht: 50-70 kDa, 33 kDa
• GenBank-Zugangsnummer: BC020843
• Gene symbol: HAVCR2
• Gene ID (NCBI): 84868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301)

Publikationen

Species	Application	Title
mouse	WB	Front Mol Neurosci; Galectin-9/Tim-3 pathway mediates dopaminergic neurodegeneration in MPTP-induced mouse model of Parkinson’s disease; Authors - Qinyu Peng
human	WB	Front Cell Infect Microbiol; The Role of Tim-3 on dNK Cells Dysfunction During Abnormal Pregnancy With Toxoplasma gondii Infection.; Authors - Teng Li
human	IHC	CNS Neurosci Ther; PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma.; Authors - Hao Zhang
		Int J Oncol; Role of downregulation of galectin-9 in the tumorigenesis of gastric cancer.; Authors - Jiaxing Yang
mouse	WB	Iran J Immunol; The Immunomodulatory Effects and Mechanisms of Tim-3 Action in the Early Stage of Mice with Severe Acute Pancreatitis.; Authors - Min Lin
human	WB	Immun Inflamm Dis; Galectin-9 and myeloid-derived suppressor cell as prognostic indicators for chronic lymphocytic leukemia; Authors - Xierenguli Alimu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Qing (Verified Customer) (06-10-2022)

It work nicely

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse

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