HAX1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HAX1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, IF/ICC, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Rezensionen (1)

Kat-Nr. : 11266-1-AP

Synonyme

HAX 1, HAX-1, HCLS1-associated protein X-1, HCLSBP1, HS1-associating protein X-1

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  WB analysis of HeLa using 11266-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11266-1-AP (HAX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11266-1-AP

  WB result of HAX1 antibody (11266-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAX1 transfected HEK-293 cells.

• 

  WB analysis of HeLa using 11266-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11266-1-AP (HAX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11266-1-AP

  IP result of anti-HAX1 (IP:11266-1-AP, 3ug; Detection:11266-1-AP 1:300) with mouse brain tissue lysate 6000ug.

• 

  IHC staining of human lymphoma using 11266-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11266-1-AP (HAX1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 11266-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11266-1-AP (HAX1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11266-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11266-1-AP (HAX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11266-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HAX1 antibody (11266-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 16 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• IP: See 3 publications below
• RIP: See 1 publications below

Produktinformation

11266-1-AP bindet in WB, IHC, IF/ICC, IP, RIP, ELISA HAX1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe, hamster, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HAX1 fusion protein Ag1792
• Vollständiger Name: HCLS1 associated protein X-1
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC014314
• Gene symbol: HAX1
• Gene ID (NCBI): 10456
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HAX1, also named as HS1BP1, is a 32 kDa protein that is ubiquitously expressed. Hax1 has been demonstrated to be a negative regulator of apoptosis in many immune cell types. Furthermore, Hax1 has been shown to have additional roles in regulating cell motility and adhesion, and is overexpressed in many types of cancer. Kristina and Cavnar demonstrate in their paper validation of two research antibodies including Proteintech's anti-Hax1 (11266-1-AP). They have evidence that the rabbit anti-Hax1 (11266-1-AP, Proteintech Group Inc.) results in a more robust and consistent detection of Hax1.

Publikationen

Species	Application	Title
human	WB	Mol Cell; The IMiD target CRBN determines HSP90 activity toward transmembrane proteins essential in multiple myeloma.; Authors - Michael Heider
hamster	WB,IP	Nat Commun; Cerebellar Kv3.3 potassium channels activate TANK-binding kinase 1 to regulate trafficking of the cell survival protein Hax-1.; Authors - Yalan Zhang
human	WB	J Cell Biol; Hax1 regulates neutrophil adhesion and motility through RhoA.; Authors - Cavnar Peter J PJ ; KD Validated
mouse	WB	FASEB J; Suppression of Kv3.3 channels by antisense oligonucleotides reverses biochemical effects and motor impairment in spinocerebellar ataxia type 13 mice.; Authors - Yalan Zhang
human	WB,IHC	J Cell Mol Med; HAX1 maintains the glioma progression in hypoxia through promoting mitochondrial fission.; Authors - Jinghui Lin
human	WB	Fish Shellfish Immunol; Lamprey PHB2 maintains mitochondrial stability by tanslocation to the mitochondria under oxidative stress.; Authors - Ying Shi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sara (Verified Customer) (10-06-2024)

It works well for immunofluorescence. I stained mouse primary lymphocytes with it and gave a nice staining, it seems to localise to the mitochondria as described in the literature.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:150
• Cell Tissue Type: Mouse primary lymphocytes

HAX1 Antibody Immunofluorescence validation (1:150 dilution) in Mouse primary lymphocytes (Cat no:11266-1-AP)