Hemoglobin Alpha Rekombinanter Antikörper

Hemoglobin Alpha Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240049F12

Kat-Nr. : 83185-5-PBS

Synonyme

HBA1, Hemoglobin a, HBA2, Alpha-globin, Alpha globin

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  WB analysis of human placenta using 83185-5-RR (same clone as 83185-5-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 83185-5-RR (HBA1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83185-5-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 83185-5-RR (same clone as 83185-5-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug Hba1 Recombinant Antibody (83185-5-RR, Clone:240049F12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83185-5-PBS in a different storage buffer formulation.

• 

  FC experiment of TF-1 using 83185-5-RR (same clone as 83185-5-PBS)

  1x10^6 TF-1 cells were intracellularly stained with 0.25 ug Hba1 Recombinant Antibody (83185-5-RR, Clone:240049F12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83185-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83185-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83185-5-RR against Human HBA1 were performed. The affinity constant is 3.94 nM.

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Geprüfte Anwendungen

Produktinformation

83185-5-PBS bindet in WB, FC (Intra), ELISA Hemoglobin Alpha und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Hemoglobin Alpha fusion protein Ag6040
• Vollständiger Name: hemoglobin, alpha 1
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 14-16 kDa, 27 kDa
• GenBank-Zugangsnummer: BC050661
• Gene symbol: HBA1
• Gene ID (NCBI): 3039
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). This antibody may cross-react with other hemoglobin chains.