HBB Polyklonaler Antikörper

HBB Polyklonal Antikörper für IF-P, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF-P, FC (Intra)

Konjugation

CoraLite® Plus 750 Fluorescent Dye

Kat-Nr. : CL750-16216

Synonyme

beta globin, Beta-globin, Hemoglobin beta chain, Hemoglobin subunit beta, LVV-hemorphin-7

---

• 

  IF Staining of human appendicitis using CL750-16216

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite® Plus 750 HBB antibody (CL750-16216) at dilution of 1:200.

• 

  IF Staining of human placenta using CL750-16216

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite® Plus 750 HBB antibody (CL750-16216) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of K-562 using CL750-16216

  1X10^6 K-562 cells were intracellularly stained with 0.8 ug CoraLite® Plus 750 Anti-Human HBB (CL750-16216) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Appendizitis-Gewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in FC (Intra): K-562-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL750-16216 bindet in IF-P, FC (Intra) HBB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HBB fusion protein Ag9216
• Vollständiger Name: hemoglobin, beta
• Berechnetes Molekulargewicht: 147 aa, 16 kDa
• Beobachtetes Molekulargewicht: 13-16 kDa
• GenBank-Zugangsnummer: BC007075
• Gene symbol: HBB
• Gene ID (NCBI): 3043
• Konjugation: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBB gene encodes hemoglobin beta chain. The hemoglobin chains, each with its own heme moiety, cooperate in binding and release of oxygen. Hemoglobin monomers have been found in blood and other tissues including macrophages, alveolar epithelial cells and brain. Defects in HBB have been linked to diseases including Sickle cell anemia, Beta-thalassemia, and Heinz body anemias. This antibody, raised against the full-length human HBB, recognizes the 13-16 kDa HBB in western blot analyses of human heart and placenta.