HBG1/2-Specific Polyklonaler Antikörper

HBG1/2-Specific Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19993-1-AP

Synonyme

Gamma 1 globin, Hb F Agamma, HBG1, HBG1-Specific, HBGA, HBGR, Hemoglobin gamma 1 chain, Hemoglobin gamma A chain, Hemoglobin subunit gamma 1, hemoglobin, gamma A, HSGGL1, PRO2979

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Galerie der Validierungsdaten

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  WB analysis of recombinant proein using 19993-1-AP

  WB results of 19993-1-AP (HBG1/2-Specific antibody) at dilution of 1:2000.

• 

  WB analysis of human placenta using 19993-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19993-1-AP (HBG1/2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using 19993-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human HBG1/2-Specific (19993-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: recombinant proein, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in FC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19993-1-AP bindet in WB, FC, ELISA HBG1/2-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: hemoglobin, gamma A
• Berechnetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: NM_000559
• Gene symbol: HBG1
• Gene ID (NCBI): 3047
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HBG1, also named as Hb F Agamma, HBGA, HBGR and HSGGL, belongs to the globin family. HBG1 makes up the fetal hemoglobin F, in combination with alpha chains. Some gamma variants can cause severe jaundice and cyanosis in premature and new born babies. The antibody is specific to HBG1 and HBG2. The antibody has no cross reaction to HBB and HBD. HBG1 protein migrates around 16 kDa. Heterodimer of 32 kDa may also be observed. This antibody could identify HBG1 and HBG2.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C