HBP1 Polyklonaler Antikörper

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• KD/KO Validated

HBP1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Rezensionen (1)

Kat-Nr. : 11746-1-AP

Synonyme

HBP1, HMG box containing protein 1, HMG box transcription factor 1

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Galerie der Validierungsdaten

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  WB analysis using 11746-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 11746-1-AP (HBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11746-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11746-1-AP (HBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11746-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11746-1-AP (HBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11746-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11746-1-AP (HBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11746-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11746-1-AP (HBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11746-1-AP

  IP Result of anti-HBP1 (IP:11746-1-AP, 4ug; Detection:11746-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, SGC-7901-Zellen
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

11746-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA HBP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HBP1 fusion protein Ag2342
• Vollständiger Name: HMG-box transcription factor 1
• Berechnetes Molekulargewicht: 514 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC022329
• Gene symbol: HBP1
• Gene ID (NCBI): 26959
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HMG-box protein 1 (HBP1) is a transcriptional repressor, and has a role in cell cycle progression and tumor suppression. Also it's a repressor of the cyclin D1 gene and inhibits the Wnt signaling pathway. Interaction with RB1 enhances it's binding to the H1F0 promoter . Disrupts the interaction between DNA and TCF4. This is a rabbit polyclonal antibody raised against part of C-terminus of HBP1 of human origin.

Publikationen

Species	Application	Title
human	WB	Cancer Cell; MYC through miR-17-92 suppresses specific target genes to maintain survival, autonomous proliferation, and a neoplastic state.; Authors - Yulin Li
human,mouse	WB,IHC	Pharmacol Res; Targeting of HBP1/TIMP3 axis as a novel strategy against breast cancer; Authors - Yue Zhou
mouse,human	WB,IHC	Oncogene; miR-29c&b2 encourage extramedullary infiltration resulting in the poor prognosis of acute myeloid leukemia.; Authors - Yanyu Wei ; KD Validated
human	WB,IHC	Oncogene; CRISPR interference and activation of the microRNA-3662-HBP1 axis control progression of triple-negative breast cancer.; Authors - Baozhu Yi
human	WB	EMBO Rep; The human GID complex engages two independent modules for substrate recruitment.; Authors - Weaam I Mohamed
mouse	WB	J Cell Physiol; Transcription factor HMG box-containing protein 1 (HBP1) modulates mitotic clonal expansion (MCE) during adipocyte differentiation.; Authors - Chien-Yi Chan ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

S (Verified Customer) (11-22-2021)

This antibody gave a strong one band signal.

• Applications: Western Blot