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HBQ1 Rekombinanter Antikörper

HBQ1 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240527D6

Kat-Nr. : 83576-4-PBS

Synonyme

240527D6, Hemoglobin subunit theta 1, Hemoglobin subunit theta-1, Hemoglobin theta 1 chain, Hemoglobin theta-1 chain

Filter:
  • Western Blot (WB) analysis of K-562 cells using HBQ1 Recombinant monoclonal antibody (83576-4-RR)

    WB analysis of K-562 using 83576-4-RR (same clone as 83576-4-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 83576-4-RR (HBQ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83576-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using HBQ1 Recombinant monoclonal antibody (83576-4-RR)

    IF Staining of K-562 using 83576-4-RR (same clone as 83576-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using HBQ1 antibody (83576-4-RR, Clone: 240527D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83576-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83576-4-PBS

    Affinity and Kinetic Characterization of 83576-4-PBS

    Biolayer interferometry (BLl) kinetic assay of 83576-4-PBS against Human HBQ1 was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 83576-4-RR

    Affinity and Kinetic Characterization of 83576-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83576-4-RR against Human HBQ1 were performed. The affinity constant is 73.4 pM.

Geprüfte Anwendungen

Produktinformation

83576-4-PBS bindet in WB, IF/ICC, ELISA HBQ1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen HBQ1 fusion protein Ag10105
Vollständiger Name hemoglobin, theta 1
Berechnetes Molekulargewicht 142 aa, 16 kDa
Beobachtetes Molekulargewicht10~16 kDa
GenBank-ZugangsnummerBC056686
Gene symbol HBQ1
Gene ID (NCBI) 3049
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HBQ1 (Hemoglobin subunit theta-1) is a member of the human alpha-globin gene cluster. HBQ1 mRNA is found in human fetal erythroid tissue but not in adult erythroid or other nonerythroid tissue (PMID: 3657976). HBQ1 gene was transcribed in an erythroleukemia cell line. HBQ1 has transcriptionally active and may be expressed in early erythroid tissue (PMID: 3422341).