HBQ1 Rekombinanter Antikörper

HBQ1 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240527D6

Kat-Nr. : 83576-4-RR

Synonyme

240527D6, Hemoglobin subunit theta 1, Hemoglobin subunit theta-1, Hemoglobin theta 1 chain, Hemoglobin theta-1 chain

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  WB analysis of K-562 using 83576-4-RR

  K-562 cells were subjected to SDS PAGE followed by western blot with 83576-4-RR (HBQ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of K-562 using 83576-4-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using HBQ1 antibody (83576-4-RR, Clone: 240527D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83576-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83576-4-RR against Human HBQ1 were performed. The affinity constant is 73.4 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen
• Erfolgreiche Detektion in IF/ICC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83576-4-RR bindet in WB, IF/ICC, ELISA HBQ1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HBQ1 fusion protein Ag10105
• Vollständiger Name: hemoglobin, theta 1
• Berechnetes Molekulargewicht: 142 aa, 16 kDa
• Beobachtetes Molekulargewicht: 10~16 kDa
• GenBank-Zugangsnummer: BC056686
• Gene symbol: HBQ1
• Gene ID (NCBI): 3049
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HBQ1 (Hemoglobin subunit theta-1) is a member of the human alpha-globin gene cluster. HBQ1 mRNA is found in human fetal erythroid tissue but not in adult erythroid or other nonerythroid tissue (PMID: 3657976). HBQ1 gene was transcribed in an erythroleukemia cell line. HBQ1 has transcriptionally active and may be expressed in early erythroid tissue (PMID: 3422341).