HBS1L Monoklonaler Antikörper

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• KD/KO Validated

HBS1L Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A10C4

Kat-Nr. : 68146-1-Ig

Synonyme

DKFZp686L13262, EF 1a, ERFS, HBS1, HBS1 like (S. cerevisiae), HBS1 like protein, HBS1L, HSPC276, KIAA1038

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  WB analysis using 68146-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68146-1-Ig (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 68146-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68146-1-Ig (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 68146-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68146-1-Ig (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 68146-1-Ig

  WB result of HBS1L antibody (68146-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HBS1L transfected HEK-293 cells.

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  IF Staining of U2OS using 68146-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using HBS1L antibody (68146-1-Ig, Clone: 1A10C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of U2OS using 68146-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using HBS1L antibody (68146-1-Ig, Clone: 1A10C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NCI-H1299-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68146-1-Ig bindet in WB, IF/ICC, ELISA HBS1L und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HBS1L fusion protein Ag0388
• Vollständiger Name: HBS1-like (S. cerevisiae)
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC001465
• Gene symbol: HBS1L
• Gene ID (NCBI): 10767
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HBS1L (HBS1-like), also known as EF-1a or ERFS, is a 684 amino acid protein that belongs to the GTP-binding elongation factor family and exists as multiple alternatively spliced isoforms. Expressed in kidney, brain, heart, placenta, liver, muscle and pancreas, HSB1L is thought to play a role in controlling fetal hemoglobin levels, specifically influencing platelet, monocyte and erythrocyte hemoglobin content. The gene encoding HBS1L maps to a locus on human chromosome 6 that is associated with sickle cell anemia and beta-thalassemia, suggesting a role for HBS1L in the pathogenesis of blood disorders.This antibody specifically recognizes the 75kd human HBS1L protein.