HBXIP Polyklonaler Antikörper

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• KD/KO Validated

HBXIP Polyklonal Antikörper für WB, IHC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 14492-1-AP

Synonyme

HBV X interacting protein, HBV X-interacting protein, HBX interacting protein, HBX-interacting protein, Hepatitis B virus X-interacting protein

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  WB analysis using 14492-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14492-1-AP (HBXIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 14492-1-AP

  IP result of anti-HBXIP (IP:14492-1-AP, 4ug; Detection:14492-1-AP 1:300) with K-562 cells lysate 1600 ug.

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  IHC staining of human breast cancer using 14492-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14492-1-AP (HBXIP Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 14492-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14492-1-AP (HBXIP Antibody) at dilution of 1:200 (under 40x lens).

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  FC experiment of MCF-7 using 14492-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug HBXIP Polyclonal antibody (14492-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, U2OS-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 8 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

14492-1-AP bindet in WB, IHC, IF, FC (Intra), IP, ELISA HBXIP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HBXIP fusion protein Ag5894
• Vollständiger Name: hepatitis B virus x interacting protein
• Berechnetes Molekulargewicht: 10 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC062619
• Gene symbol: HBXIP
• Gene ID (NCBI): 10542
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HBXIP, also named as XIP, belongs to the HBXIP family. It is originally identified by its interaction with the C-terminus of hepatitis B virus (HBV) X protein (HBx). HBXIP is a regulator of centrosome dynamics and cytokinesis. HBXIP is able to up-regulate S100A4 though activating STAT4 and inducing DNA methylation of PTEN.

Publikationen

Species	Application	Title
human	WB	Autophagy; KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.; Authors - Guokai Yan
human	WB	Front Oncol; Germacrone Regulates HBXIP-Mediated Cell Cycle, Apoptosis and Promotes the Formation of Autophagosomes to Inhibit the Proliferation of Gastric Cancer Cells.; Authors - Xing Fang
human	WB,IP,IHC	J Biol Chem; The oncoprotein HBXIP uses two pathways to up-regulate S100A4 in promotion of growth and migration of breast cancer cells.; Authors - Liu Shuangping S
human	WB,IHC	Cancer Lett; The oncoprotein HBXIP up-regulates Skp2 via activating transcription factor E2F1 to promote proliferation of breast cancer cells.; Authors - Xu Fuqiang F ; KD Validated
human	WB,IHC	Int J Oncol; The oncoprotein hepatitis B X-interacting protein promotes the migration of ovarian cancer cells through the upregulation of S-phase kinase-associated protein 2 by Sp1.; Authors - Fuqiang Xu ; KD Validated
human	WB,IHC	Am J Cancer Res; Elevated HBXIP expression is associated with aggressive phenotype and poor prognosis in esophageal squamous cell carcinoma.; Authors - Honggang Xia