HCFC1 Polyklonaler Antikörper

HCFC1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28569-1-AP

Synonyme

HCF C-terminal chain 2, HCF C-terminal chain 1, HCF 1, HCF, CFF

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  WB analysis using 28569-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28569-1-AP (HCFC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 28569-1-AP

  IP result of anti-HCFC1 (IP:28569-1-AP, 4ug; Detection:28569-1-AP 1:20000) with HeLa cells lysate 1600 ug.

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  IHC staining of mouse liver using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 28569-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HCFC1 antibody (28569-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C2C12-Zellen, C6-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe, humanes Lungenkarzinomgewebe, humanes Urothelkarzinomgewebe, Maus-Cerebellum-Gewebe, Mauslungengewebe, Maushautgewebe, Ratten-Cerebellum-Gewebe, Rattenlungengewebe, Rattenhautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

28569-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA HCFC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HCFC1 fusion protein Ag29663
• Vollständiger Name: host cell factor C1 (VP16-accessory protein)
• Berechnetes Molekulargewicht: 209 kDa
• Beobachtetes Molekulargewicht: 300 kDa, 100-150 kDa
• GenBank-Zugangsnummer: NM_005334
• Gene symbol: HCFC1
• Gene ID (NCBI): 3054
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HCFC1 (also known as HCF-1) was discovered in studies of herpes simplex virus (HSV) transcription. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in the control of the cell cycle and transcriptional regulation during herpes simplex virus infection. HCFC1 is a large protein of ∼300 kDa and is proteolytically processed into N- and C-terminal fragments of ∼150 kDa each.(PMID: 21295698 )