HCN2 Polyklonaler Antikörper

HCN2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30946-1-PBS

Synonyme

BCNG2, BCNG-2, Brain cyclic nucleotide-gated channel 2, HAC 1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

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  WB analysis using 30946-1-AP (same clone as 30946-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30946-1-AP (HCN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30946-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 30946-1-AP (same clone as 30946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30946-1-AP (HCN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30946-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 30946-1-AP (same clone as 30946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30946-1-AP (HCN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30946-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 30946-1-AP (same clone as 30946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30946-1-AP (HCN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30946-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 30946-1-AP (same clone as 30946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30946-1-AP (HCN2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30946-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 30946-1-AP (same clone as 30946-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using HCN2 antibody (30946-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30946-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

30946-1-PBS bindet in WB, IHC, Indirect ELISA HCN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HCN2 fusion protein Ag34038
• Vollständiger Name: hyperpolarization activated cyclic nucleotide-gated potassium channel 2
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 60 kDa, 90 kDa, 120 kDa
• GenBank-Zugangsnummer: NM_001194
• Gene symbol: HCN2
• Gene ID (NCBI): 610
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HCN2, also named as BCNG2, belongs to the potassium channel HCN family. The molecular weight of immature HCN2 is 90 kDa, and the protein is about 120 kDa after n-terminal glycosylation. It has been found that 60 kDa of HCN2 protein can be identified in mouse heart samples. Although all protein isolation steps were handled on ice and in the presence of protease inhibitors to reduce protease-dependent effects and to minimize protein degradation, it is certainly possible that the presence of the 60 kDa HCN2 protein in the heart samples does, in fact, reflect protein degradation. (PMID:21796099, PMID: 19574228)