HCN2 Rekombinanter Antikörper

HCN2 Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241601E10

Kat-Nr. : 84308-3-PBS

Synonyme

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2, HAC 1, Brain cyclic nucleotide-gated channel 2, BCNG-2, BCNG2

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  WB analysis using 84308-3-RR (same clone as 84308-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84308-3-RR (HCN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84308-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00160-2

  Sandwich ELISA standard curve of MP00160-2, Human HCN2 Recombinant Matched Antibody Pair - PBS only. 84308-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34038. 84308-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

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  Sample test of MP00160-2

  The mean HCN2 concentration was determined to be 9,765.2 ng/mL in rat brain tissue extract based on a 1.6 mg/mL extract load and 23,767.3 ng/mL in mouse brain tissue extract based on a 2.0 mg/mL extract load.

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  Affinity and Kinetic Characterization of 84308-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84308-3-RR against Human HCN2 were performed. The affinity constant is 0.795 nM.

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Geprüfte Anwendungen

Produktinformation

84308-3-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test HCN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HCN2 fusion protein Ag34038
• Vollständiger Name: hyperpolarization activated cyclic nucleotide-gated potassium channel 2
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: NM_001194
• Gene symbol: HCN2
• Gene ID (NCBI): 610
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HCN2, also named as BCNG2, belongs to the potassium channel HCN family. The molecular weight of immature HCN2 is 90 kDa, and the protein is about 120 kDa after n-terminal glycosylation. It has been found that 60 kDa of HCN2 protein can be identified in mouse heart samples. Although all protein isolation steps were handled on ice and in the presence of protease inhibitors to reduce protease-dependent effects and to minimize protein degradation, it is certainly possible that the presence of the 60 kDa HCN2 protein in the heart samples does, in fact, reflect protein degradation. (PMID:21796099, PMID: 19574228)