HCN4 Polyklonaler Antikörper

HCN4 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 55224-1-AP

Synonyme

HCN4

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Galerie der Validierungsdaten

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  WB analysis using 55224-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55224-1-AP (HCN4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

55224-1-AP bindet in WB, ELISA HCN4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: hyperpolarization activated cyclic nucleotide-gated potassium channel 4
• Berechnetes Molekulargewicht: 129 kDa
• Beobachtetes Molekulargewicht: 140-160 kDa
• GenBank-Zugangsnummer: NM_005477
• Gene symbol: HCN4
• Gene ID (NCBI): 10021
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HCN4 belongs to the potassium channel HCN family. It hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. HCN4 may contribute to the native pacemaker currents in heart (If) and in neurons (Ih). It is activated by cAMP. HCN4 may mediate responses to sour stimuli. (PMID:10228147). Defects in HCN4 are a cause of sick sinus syndrome type 2 (SSS2). Defects in HCN4 are the cause of Brugada syndrome type 8 (BRS8). This antibody is specific to HCN4.

Publikationen

Species	Application	Title
hamster	WB	Proc Natl Acad Sci U S A; A gain-of-function HCN4 mutant in the HCN domain is responsible for inappropriate sinus tachycardia in a Spanish family; Authors - Anabel Cámara-Checa
human	WB	iScience; Engineering of thick human functional myocardium via static stretching and electrical stimulation.; Authors - Danielle Pretorius
rat	WB	Bull Exp Biol Med; Glucocorticoid Receptor/HCN4 Channels Interaction in Spinal Dorsal Horn Participates in the Regulation of Neuropathic Pain after Peripheral Nerve Injury in Rats; Authors - L Zhengshuai
rat	WB	Mol Med Rep; G9a inhibition promotes the formation of pacemaker-like cells by reducing the enrichment of H3K9me2 in the HCN4 promoter region; Authors - Pei Xu
rat	WB	PLoS One; DNA methylation cooperates with H3K9me2 at HCN4 promoter to regulate the differentiation of bone marrow mesenchymal stem cells into pacemaker-like cells; Authors - XiaoLin Sun