HDAC1 Monoklonaler Antikörper

HDAC1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

CloneNo.

4C4G7

Kat-Nr. : 66085-1-Ig

Synonyme

4C4G7, EC:3.5.1.-, EC:3.5.1.98, GON 10, HD1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBLNCaP-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
Erfolgreiche Detektion in IHChumanes Lymphomgewebe, humanes Lungenkarzinomgewebe, humanes Prostatakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCA431-Zellen, HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:2000-1:10000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:6000-1:24000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66085-1-Ig bindet in WB, IHC, IF/ICC, ChIP, ELISA HDAC1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen HDAC1 fusion protein Ag19128
Vollständiger Name histone deacetylase 1
Berechnetes Molekulargewicht 55 kDa
Beobachtetes Molekulargewicht 65 kDa
GenBank-ZugangsnummerBC000301
Gene symbol HDAC1
Gene ID (NCBI) 3065
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is raised against residues near the C terminus of human HDAC1. The calculated molecular weight of HDAC1 is 55 kDa, but modified HDAC1 is about 60-65 kDa .

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for HDAC1 antibody 66085-1-IgProtokoll herunterladen
IHC protocol for HDAC1 antibody 66085-1-IgProtokoll herunterladenl
IF protocol for HDAC1 antibody 66085-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Mol Cancer

Exosomal circ_0006896 promotes AML progression via interaction with HDAC1 and restriction of antitumor immunity

Authors - Can Can
mouseWB

Immunity

Histone Deacetylases 1 and 2 Regulate Microglia Function during Development, Homeostasis, and Neurodegeneration in a Context-Dependent Manner.

Authors - Moumita Datta
humanIF

Nat Commun

YTHDF2 upregulation and subcellular localization dictate CD8 T cell polyfunctionality in anti-tumor immunity

Authors - Haiyan Zhang
humanWB

J Clin Invest

The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.

Authors - Wanjin Li
humanWB

Nat Commun

ZNF516 suppresses EGFR by targeting the CtBP/LSD1/CoREST complex to chromatin.

Authors - Lifang Li
mouseWB

Br J Pharmacol

Physalin B attenuates liver fibrosis via suppressing LAP2α-HDAC1-mediated deacetylation of the transcription factor GLI1 and hepatic stellate cell activation.

Authors - Xiaoyun Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Tsimafei (Verified Customer) (08-04-2024)

Antibody were incubated at 4C, ON

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: mESCs
HDAC1 Antibody Western Blot validation (1:10000 dilution) in mESCs (Cat no:66085-1-Ig)
FH

Xiaoyu (Verified Customer) (06-28-2023)

Excellent antibody for WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: 293T
HDAC1 Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:66085-1-Ig)
FH

Andrea (Verified Customer) (01-11-2023)

Excellent antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: mESCs
HDAC1 Antibody Western Blot validation (1:5000 dilution) in mESCs (Cat no:66085-1-Ig)