HDAC1 Monoklonaler Antikörper

HDAC1 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4C4G7

Kat-Nr. : CL488-66085

Synonyme

DKFZp686H12203, GON 10, HD1, HDAC1, histone deacetylase 1, RPD3, RPD3L1

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Galerie der Validierungsdaten

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  IF Staining of A431 using CL488-66085

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 HDAC1 antibody (CL488-66085, Clone: 4C4G7 ) at dilution of 1:2000.

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  IF Staining of A431 using CL488-66085

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 HDAC1 antibody (CL488-66085, Clone: 4C4G7 ) at dilution of 1:2000, CL594-Phalloidin (red).

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  FC experiment of HeLa using CL488-66085

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human HDAC1 (CL488-66085, Clone:4C4G7) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: A431-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:1000-1:4000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66085 bindet in IF, FC (Intra) HDAC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HDAC1 fusion protein Ag19128
• Vollständiger Name: histone deacetylase 1
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC000301
• Gene symbol: HDAC1
• Gene ID (NCBI): 3065
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is raised against residues near the C terminus of human HDAC1. The calculated molecular weight of HDAC1 is 55 kDa, but modified HDAC1 is about 60-65 kDa .

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011