HDAC10 Monoklonaler Antikörper

HDAC10 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1A1B11

Kat-Nr. : 67646-1-Ig

Synonyme

1A1B11, EC:3.5.1.48, HD 10, HD10, HDAC 10

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  WB analysis using 67646-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67646-1-Ig (HDAC10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 67646-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67646-1-Ig (HDAC10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 67646-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using HDAC10 antibody (67646-1-Ig, Clone: 1A1B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of MCF-7 using 67646-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using HDAC10 antibody (67646-1-Ig, Clone: 1A1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67646-1-Ig bindet in WB, IF/ICC, ELISA HDAC10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HDAC10 fusion protein Ag18470
• Vollständiger Name: histone deacetylase 10
• Berechnetes Molekulargewicht: 669 aa, 71 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC125083
• Gene symbol: HDAC10
• Gene ID (NCBI): 83933
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HDAC10, also named as Histone deacetylase 10, is a 669 amino acid protein, which is widely expressed with high levels in liver and kidney. HDAC10, which belongs to the class IIb subfamily, has an active deacetylase domain (DAC) and a catalytically inactive leucine-rich domain (LRD). HDAC10 is determined that it plays a role in homologous recombination, promotes autophagy and survival in neuroblastoma cells, suppresses cervical cancer metastasis and facilitates the cell cycle.

Publikationen

Species	Application	Title
human	WB	Transl Cancer Res; Molecular, biological characterization and drug sensitivity of chidamide-resistant MCF7 cells; Authors - Liya Dai