HDAC2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

HDAC2 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

1A3E4

Rezensionen (2)

Kat-Nr. : 67165-1-Ig

Synonyme

HD2, HDAC, HDAC2, histone deacetylase 2, RPD3, YAF1

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  WB analysis using 67165-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67165-1-Ig (HDAC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 67165-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67165-1-Ig (HDAC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67165-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67165-1-Ig (HDAC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67165-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67165-1-Ig (HDAC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67165-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDAC2 antibody (67165-1-Ig, Clone: 1A3E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 67165-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human HDAC2 (67165-1-Ig, Clone:1A3E4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

67165-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA HDAC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HDAC2 fusion protein Ag21288
• Vollständiger Name: histone deacetylase 2
• Berechnetes Molekulargewicht: 458 aa, 52 kDa; 488 aa,55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC031055
• Gene symbol: HDAC2
• Gene ID (NCBI): 3066
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is raised against residues near the C terminus of human HDAC2.

Publikationen

Species	Application	Title
human	WB,CoIP	iScience; Modification of lysine-260 2-hydroxyisobutyrylation destabilizes ALDH1A1 expression to regulate bladder cancer progression; Authors - Zhilei Zhang ; KD Validated
human	WB	Molecules; Anti-Colorectal Cancer Activity of Solasonin from Solanum nigrum L. via Histone Deacetylases-Mediated p53 Acetylation Pathway; Authors - Xintian Lan
human,rat	WB,IF,IP	Toxicol Appl Pharmacol; Advanced oxidation protein products upregulate ABCB1 expression and activity via HDAC2-Foxo3α-mediated signaling in vitro and in vivo.; Authors - Tianrong Xun ; KD Validated
human	WB,CoIP	Theranostics; CRISPR screening reveals ZNF217 as a vulnerability in high-risk B-cell acute lymphoblastic leukemia; Authors - Xi Qin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xinda (Verified Customer) (02-24-2025)

human iPSC derived NPC cell lysates were used as input. Co-immunoprecipitation was performed against a protein of interest. On the graph, one can see that the beads coupled with control antibody did not pull out HDAC2, whereas the protein of interest pulled out HDAC2. The groups are not labeled (because our results are not published), but in triplicate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

HDAC2 Antibody Western Blot validation (1:1000 dilution) in (Cat no:67165-1-Ig)

FH

Xiaoyu (Verified Customer) (06-28-2023)

Good for wb

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

HDAC2 Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:67165-1-Ig)