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HDAC2 Polyklonaler Antikörper

HDAC2 Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-12922

Synonyme

HD2, HDAC2, histone deacetylase 2, RPD3, YAF1



Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen
Erfolgreiche Detektion in FCHEK-293T-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-12922 bindet in IF, FC (Intra) HDAC2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HDAC2 fusion protein Ag3607
Vollständiger Name histone deacetylase 2
Berechnetes Molekulargewicht458 aa, 52 kDa; 488 aa,55 kDa
Beobachtetes Molekulargewicht 55-60 kDa
GenBank-ZugangsnummerBC031055
Gene symbol HDAC2
Gene ID (NCBI) 3066
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human HDAC2.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 HDAC2 antibody CL488-12922Protokoll herunterladen
FC protocol for CL Plus 488 HDAC2 antibody CL488-12922Protokoll herunterladen
Standard-Protokolle
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