HDAC2-specific Polyklonaler Antikörper

HDAC2-specific Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 16152-1-AP

Synonyme

HD2, HDAC2, HDAC2-specific, histone deacetylase 2, RPD3, YAF1

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 16152-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16152-1-AP (HDAC2-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 16152-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16152-1-AP (HDAC2-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16152-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16152-1-AP (HDAC2-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16152-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16152-1-AP (HDAC2-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 16152-1-AP

  IP Result of anti-HDAC2-specific (IP:16152-1-AP, 4ug; Detection:16152-1-AP 1:500) with A431 cells lysate 2400ug.

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  IHC staining of human testis using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 16152-1-AP

  Immunofluorescent analysis of HeLa cells using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A431-Zellen, HeLa-Zellen, humanes Lebergewebe
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Hirngewebe, humanes Lebergewebe, humanes Milzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

16152-1-AP bindet in WB, IP, IHC, IF, ELISA HDAC2-specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone deacetylase 2
• Berechnetes Molekulargewicht: 458 aa, 52 kDa; 488 aa, 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: NM_001527
• Gene symbol: HDAC2
• Gene ID (NCBI): 3066
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human HDAC2 and is specific to HDAC2. It will not cross react with other HDACs.

Publikationen

Species	Application	Title
human		Hum Reprod; Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility.; Authors - Yi-Min Cheng
human	WB	Chemosphere; Combined exposure of lead and cadmium leads to the aggravated neurotoxicity through regulating the expression of histone deacetylase 2.; Authors - Ruiqing Zhou
human	IF	Leuk Res; High-expressing cystic fibrosis transmembrane conductance regulator interacts with histone deacetylase 2 to promote the development of Ph+ leukemia through the HDAC2-mediated PTEN pathway.; Authors - Tianyou Yan
human	WB	Mol Cell; Histone H3 lysine 27 crotonylation mediates gene transcriptional repression in chromatin; Authors - Nan Liu