HDAC3 Polyklonaler Antikörper

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• KD/KO Validated

HDAC3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, CoIP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

44

Kat-Nr. : 10255-1-AP

Synonyme

HD3, HDAC3, histone deacetylase 3, RPD3, RPD3 2, SMAP45

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Galerie der Validierungsdaten

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  WB analysis of U-251 using 10255-1-AP

  WB result of HDAC3 antibody (10255-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDAC3 transfected U-251 cells.

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  WB analysis using 10255-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10255-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 10255-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10255-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10255-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 10255-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 10255-1-AP

  IP Result of anti-HDAC3 (IP:10255-1-AP, 3ug; Detection:10255-1-AP 1:300) with A431 cells lysate 1200ug.

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  IHC staining of human ovary tumor using 10255-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10255-1-AP (HDAC3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 10255-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10255-1-AP (HDAC3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of K-562 using 10255-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using HDAC3 antibody (10255-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen, U-251-Zellen
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 37 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below

Produktinformation

10255-1-AP bindet in WB, IP, IHC, IF, CoIP, chIP, ELISA HDAC3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HDAC3 fusion protein Ag0396
• Vollständiger Name: histone deacetylase 3
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC000614
• Gene symbol: HDAC3
• Gene ID (NCBI): 8841
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

What is the molecular weight of HDAC3? Is HDAC3 post-translationally modified?

The molecular weight of HDAC3 is 49 kDa but HDAC3 can form homodimers and homotrimers in cells (PMID: 11779848). HDAC3 can be phosphorylated at S424 residue by protein kinase CK2, which regulates HDAC3 activity (PMID: 15805470).

 What is the subcellular localization of HDAC3?

Unlike other class I HDACs, HDAC3 actively shuttles between the nucleus and cytoplasm (PMID: 11779848). Additionally, HDAC3 can be present at the plasma membrane, where it associates with c-Src (PMID: 16532030).

 What is the tissue expression pattern of HDAC3?

Like other class I HDACs, HDAC3 is ubiquitously expressed (PMID: 9346952).

 What is the mechanism of HDAC3 in regulating gene expression?

Unlike other histone deacetylates, it exists in a complex with two proteins - nuclear receptor co-repressor (N-CoR) and silencing mediator for retinoid and thyroid receptors (SMRT) - and therefore acts as a mediator of the repression function of unliganded nuclear hormone receptors (PMID: 10809664). N-CoR and SMRT play an active role in the recruitment of HDAC3 to binding sites as well as directly regulating HDAC3 activity. Additionally, HDAC3 targets growth-regulated genes.

Publikationen

Species	Application	Title
human	WB	Acta Pharm Sin B; Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy.; Authors - Xin Sun
mouse	WB	J Clin Invest; Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.; Authors - Shanshan Zhong
human	WB	Mol Ther; Genetic and Pharmacological Inhibition of GRPR Protects Against Acute Kidney Injury via Attenuating Renal Inflammation and Necroptosis; Authors - Chao Li
mouse	WB	J Exp Med; Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis.; Authors - Zhe Chen
rat	WB	Cell Rep; Gut microbiota shapes social dominance through modulating HDAC2 in the medial prefrontal cortex.; Authors - Tian Wang
		Oncogene; miR-155 downregulates ErbB2 and suppresses ErbB2-induced malignant transformation of breast epithelial cells.; Authors - X-H He