HDAC3 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

HDAC3 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2N1

Kat-Nr. : 81211-1-PBS

Synonyme

2N1, EC:3.5.1.-, EC:3.5.1.98, HDAC 3, Histone deacetylase 3

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  WB analysis using 81211-1-RR (same clone as 81211-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81211-1-RR (HDAC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HAP1 using 81211-1-RR (same clone as 81211-1-PBS)

  WB result of HDAC3 antibody (81211-1-RR; 1:2000; room temperature for 1.5 hours) with wild-type and sg-HDAC3 transfected HAP1 cells This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 81211-1-RR (same clone as 81211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 81211-1-RR (same clone as 81211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 81211-1-RR (same clone as 81211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 81211-1-RR (same clone as 81211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 81211-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81211-1-RR against Human HDAC3 were performed. The affinity constant is 0.213 nM.

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Geprüfte Anwendungen

Produktinformation

81211-1-PBS bindet in WB, IHC, Indirect ELISA HDAC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HDAC3 fusion protein Ag0396
• Vollständiger Name: histone deacetylase 3
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC000614
• Gene symbol: HDAC3
• Gene ID (NCBI): 8841
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the (-amino groups of lysines located near the amino termini of core histone proteins. At least 4 classes of HDAC were identified. HDAC3 is a class I HDAC. HDAC3 has histone deacetylase activity and may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. HDAC3 can also down-regulate p53 function and thus modulate cell growth and apoptosis. The gene encoding HDAC3 is regarded as a potential tumor suppressor gene.