HDAC4 Polyklonaler Antikörper

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• KD/KO Validated

HDAC4 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

29

Kat-Nr. : 17449-1-AP

Synonyme

HA6116, HD4, HDAC A, HDAC4, HDACA, histone deacetylase 4, KIAA0288

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Galerie der Validierungsdaten

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  WB analysis using 17449-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17449-1-AP (HDAC4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17449-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17449-1-AP (HDAC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17449-1-AP

  IP Result of anti-HDAC4 (IP:17449-1-AP, 4ug; Detection:17449-1-AP 1:500) with HeLa cells lysate 4000ug.

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  IHC staining of human kidney using 17449-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17449-1-AP (HDAC4 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human cervical cancer using 17449-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17449-1-AP (HDAC4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 17449-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17449-1-AP (HDAC4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 17449-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17449-1-AP (HDAC4 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293T-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 23 publications below
• IHC: See 5 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Produktinformation

17449-1-AP bindet in WB, IP, IHC, IF, CoIP, ChIP, ELISA HDAC4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HDAC4 fusion protein Ag11572
• Vollständiger Name: histone deacetylase 4
• Berechnetes Molekulargewicht: 972 aa, 106 kDa
• Beobachtetes Molekulargewicht: 119-140 kDa
• GenBank-Zugangsnummer: BC039904
• Gene symbol: HDAC4
• Gene ID (NCBI): 9759
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. This antibody is a rabbit polyclonal antibody raised against an internal region of human HDAC4. The calculated molecular weight of HDAC4 is 106 kDa, but modified HDAC4 is about 140 kDa.

Publikationen

Species	Application	Title
	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang ; KD Validated
human	WB, IF	Hepatology; Histone Deacetylase 4 promotes cholestatic liver injury in the absence of Prohibitin-1.; Authors - Lucía Barbier-Torres ; KD Validated
	WB,IHC,IF	Kidney Int; Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.; Authors - Xiaojie Wang ; KD Validated
human	ChIP,CoIP	Mol Ther Nucleic Acids; Transcriptional Factor Yin Yang 1 Promotes the Stemness of Breast Cancer Cells by Suppressing miR-873-5p Transcriptional Activity.; Authors - Qianqian Guo
human	WB	J Cell Mol Med; Low levels of AMPK promote epithelial-mesenchymal transition in lung cancer primarily through HDAC4- and HDAC5-mediated metabolic reprogramming.; Authors - Shoujie Feng ; KD Validated
human	WB	J Am Heart Assoc; Phosphatidylinositol 3-Kinase-DNA Methyltransferase 1-miR-1281-Histone Deacetylase 4 Regulatory Axis Mediates Platelet-Derived Growth Factor-Induced Proliferation and Migration of Pulmonary Artery Smooth Muscle Cells.; Authors - Yanjiao Li