HDAC4-specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HDAC4-specific Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 16165-1-AP

Synonyme

HDAC4, KIAA0288, HD4, EC:3.5.1.98

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  WB analysis using 16165-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16165-1-AP (HDAC4-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16165-1-AP

  IP result of anti-HDAC4-specific (IP:16165-1-AP, 4ug; Detection:16165-1-AP 1:500) with HeLa cells lysate 2440ug.

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  IHC staining of human ovary cancer using 16165-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 16165-1-AP (HDAC4-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16165-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16165-1-AP (HDAC4-specific Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary cancer using 16165-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 16165-1-AP (HDAC4-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16165-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16165-1-AP (HDAC4-specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of mouse liver using 16165-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using HDAC4-specific antibody (16165-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

16165-1-AP bindet in WB, IHC, IF-P, IP, ELISA HDAC4-specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone deacetylase 4
• Berechnetes Molekulargewicht: 119 kDa
• Beobachtetes Molekulargewicht: 119-140 kDa
• GenBank-Zugangsnummer: BC039904
• Gene symbol: HDAC4
• Gene ID (NCBI): 9759
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human HDAC4.

Publikationen

Species	Application	Title
human,mouse	IF	Nat Cell Biol; Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.; Authors - Yoshihiko Kobayashi
human	WB	Hepatology; The histone deacetylase 4/SP1/microrna-200a regulatory network contributes to aberrant histone acetylation in hepatocellular carcinoma.; Authors - Yuan Ji-hang JH ; KD Validated
mouse	WB	J Orthop Translat; Irisin reduces bone fracture by facilitating osteogenesis and antagonizing TGF-β/Smad signaling in a growing mouse model of osteogenesis imperfecta; Authors - Bin Sun
mouse	WB	Biol Trace Elem Res; Exercise Alleviates Fluoride-Induced Learning and Memory Impairment in Mice: Role of miR-206-3p and PREG; Authors - Lei Chai
		Am J Physiol Lung Cell Mol Physiol; Complex urban atmosphere alters alveolar stem cells niche properties and drives lung fibrosis; Authors - Randa Belgacemi