• Featured Product
  • KD/KO Validated

HDAC5-specific Polyklonaler Antikörper

HDAC5-specific Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(27)

Kat-Nr. : 16166-1-AP

Synonyme

HDAC5, Histone deacetylase 5, HD5, EC:3.5.1.98, Antigen NY-CO-9



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, fetales humanes Hirngewebe, HEK-293-Zellen
Erfolgreiche Detektion in IHCMaushirngewebe, humanes Hirngewebe, humanes Herzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:100-1:1000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

16166-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA HDAC5-specific und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name histone deacetylase 5
Berechnetes Molekulargewicht 122 kDa
Beobachtetes Molekulargewicht120-140 kDa
GenBank-ZugangsnummerBC051824
Gene symbol HDAC5
Gene ID (NCBI) 10014
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone acetylation and deacetylation alternately expose and occlude DNA to transcription factors. At least 4 classes of HDAC were identified. HDAC5 is a class II HDAC. HDAC5 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3, and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 is involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, HDAC5 shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. This antibody only binds HDAC5. It does not cross-react with other HDACs.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for HDAC5-specific antibody 16166-1-APProtokoll herunterladen
IHC protocol for HDAC5-specific antibody 16166-1-APProtokoll herunterladenl
IF protocol for HDAC5-specific antibody 16166-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell

Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.

Authors - Xun Huang
humanWB

Sci Transl Med

ATP citrate lyase drives vascular remodeling in systemic and pulmonary vascular diseases through metabolic and epigenetic changes

Authors - Yann Grobs
mouseWB,IF

Biomaterials

Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure

Authors - Lingjun Tong
mouse, ratWB,IHC,IF

Kidney Int

Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.

Authors - Xiaojie Wang
humanWB

Cancer Lett

ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer

Authors - Chen Zhou
humanWB

J Cell Mol Med

Low levels of AMPK promote epithelial-mesenchymal transition in lung cancer primarily through HDAC4- and HDAC5-mediated metabolic reprogramming.

Authors - Shoujie Feng
  • KD Validated