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HDAC5-specific Polyklonaler Antikörper
HDAC5-specific Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus und mehr (1)
Anwendung
WB, IHC, IF/ICC, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 16166-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HeLa-Zellen, fetales humanes Hirngewebe, HEK-293-Zellen |
Erfolgreiche Detektion in IHC | Maushirngewebe, humanes Hirngewebe, humanes Herzgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | HepG2-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:100-1:1000 |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 5 publications below |
WB | See 26 publications below |
IHC | See 4 publications below |
IF | See 4 publications below |
CoIP | See 3 publications below |
Produktinformation
16166-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA HDAC5-specific und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | histone deacetylase 5 |
Berechnetes Molekulargewicht | 122 kDa |
Beobachtetes Molekulargewicht | 120-140 kDa |
GenBank-Zugangsnummer | BC051824 |
Gene symbol | HDAC5 |
Gene ID (NCBI) | 10014 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Histone acetylation and deacetylation alternately expose and occlude DNA to transcription factors. At least 4 classes of HDAC were identified. HDAC5 is a class II HDAC. HDAC5 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3, and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 is involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, HDAC5 shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. This antibody only binds HDAC5. It does not cross-react with other HDACs.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for HDAC5-specific antibody 16166-1-AP | Protokoll herunterladen |
IHC protocol for HDAC5-specific antibody 16166-1-AP | Protokoll herunterladenl |
IF protocol for HDAC5-specific antibody 16166-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Cell Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors. | ||
Sci Transl Med ATP citrate lyase drives vascular remodeling in systemic and pulmonary vascular diseases through metabolic and epigenetic changes | ||
Biomaterials Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure | ||
Kidney Int Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy. | ||
Cancer Lett ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer | ||
J Cell Mol Med Low levels of AMPK promote epithelial-mesenchymal transition in lung cancer primarily through HDAC4- and HDAC5-mediated metabolic reprogramming.
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