HDAC5-specific Polyklonaler Antikörper

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• KD/KO Validated

HDAC5-specific Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16166-1-PBS

Synonyme

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  WB analysis of HeLa using 16166-1-AP (same clone as 16166-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16166-1-AP (same clone as 16166-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 16166-1-AP (same clone as 16166-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 16166-1-AP (same clone as 16166-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 16166-1-AP (same clone as 16166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16166-1-AP (HDAC5-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 16166-1-AP (same clone as 16166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 16166-1-AP (HDAC5-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 16166-1-AP (same clone as 16166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16166-1-AP (HDAC5-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16166-1-AP (same clone as 16166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16166-1-AP (HDAC5-specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16166-1-AP (same clone as 16166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16166-1-AP (HDAC5-specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16166-1-AP (same clone as 16166-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDAC5-specific antibody (16166-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16166-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HDAC5-specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone deacetylase 5
• Berechnetes Molekulargewicht: 122 kDa
• Beobachtetes Molekulargewicht: 120-140 kDa
• GenBank-Zugangsnummer: BC051824
• Gene symbol: HDAC5
• Gene ID (NCBI): 10014
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone acetylation and deacetylation alternately expose and occlude DNA to transcription factors. At least 4 classes of HDAC were identified. HDAC5 is a class II HDAC. HDAC5 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3, and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 is involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, HDAC5 shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. This antibody only binds HDAC5. It does not cross-react with other HDACs.