HDAC6 Monoklonaler Antikörper

HDAC6 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C7C3

Kat-Nr. : 67250-1-PBS

Synonyme

1C7C3, EC:3.5.1.-, EC:3.5.1.98, HDAC 6, Histone deacetylase 6

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  WB analysis using 67250-1-Ig (same clone as 67250-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67250-1-Ig (HDAC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67250-1-Ig (same clone as 67250-1-PBS)

  WB result of HDAC6 antibody (67250-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDAC6 transfected HeLa cells. This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67250-1-Ig (same clone as 67250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67250-1-Ig (HDAC6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67250-1-Ig (same clone as 67250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67250-1-Ig (HDAC6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67250-1-Ig (same clone as 67250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67250-1-Ig (HDAC6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67250-1-Ig (same clone as 67250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67250-1-Ig (HDAC6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67250-1-Ig (same clone as 67250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67250-1-Ig (HDAC6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 67250-1-Ig (same clone as 67250-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using HDAC6 antibody (67250-1-Ig, Clone: 1C7C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67250-1-Ig (same clone as 67250-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDAC6 antibody (67250-1-Ig, Clone: 1C7C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67250-1-PBS bindet in WB, IHC, IF/ICC, ELISA HDAC6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HDAC6 fusion protein Ag28585
• Vollständiger Name: histone deacetylase 6
• Berechnetes Molekulargewicht: 1063 aa, 114 kDa, 131 kDa
• Beobachtetes Molekulargewicht: 150-160 kDa
• GenBank-Zugangsnummer: BC013737
• Gene symbol: HDAC6
• Gene ID (NCBI): 10013
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC6 is a member of the class II mammalian histone deacetylases. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. HDAC6 has some splicing variants such as P114(~130kd), P131(~160kd).This antibody is raised against residues near the C terminal of human HDAC6. The calculated molecular weight of HDAC6 is 130 kDa, but the modified the HDAC6 is about 150-160 kDa.