HDAC6-specific Polyklonaler Antikörper

HDAC6-specific Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 16167-1-AP

Synonyme

HDAC6, EC:3.5.1.-, EC:3.5.1.98, HDAC 6, Histone deacetylase 6



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMCF7 cells, HT-1080.Zellen, MCF-7-Zellen
Erfolgreiche Detektion in IHCMaushirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:200-1:1000
Immunhistochemie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

16167-1-AP bindet in WB, IHC, IF, ELISA HDAC6-specific und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte ReaktivitätMaus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name histone deacetylase 6
Berechnetes Molekulargewicht 131 kDa
Beobachtetes Molekulargewicht 130 kDa
GenBank-ZugangsnummerBC005872
Gene symbol HDAC6
Gene ID (NCBI) 10013
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC6 is a member of the class II mammalian histone deacetylases. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. This antibody is specific to HDAC6. It does not cross react with other HDACs.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for HDAC6-specific antibody 16167-1-APProtokoll herunterladen
IHC protocol for HDAC6-specific antibody 16167-1-APProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB,IHC

Oncogene

MicroRNA-22 represses glioma development via activation of macrophage-mediated innate and adaptive immune responses.

Authors - Jiajie Tu
ratWB

J Cell Mol Med

HDAC4/5-HMGB1 signalling mediated by NADPH oxidase activity contributes to cerebral ischaemia/reperfusion injury.

Authors - He Min M
mouseWB

Adv Sci (Weinh)

Arf1 GTPase Regulates Golgi-Dependent G2/M Transition and Spindle Organization in Oocyte Meiosis

Authors - Kun-Huan Zhang
mouseIF

Nucl Med Biol

Carbon-11-labelling of the histone deacetylase 6 radioligand EKZ-001

Authors - Tetsuro Tago