HDAC6-specific Polyklonaler Antikörper

HDAC6-specific Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 16167-1-AP

Synonyme

HD6, HDAC6, histone deacetylase 6, JM21, KIAA0901

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 16167-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16167-1-AP (HDAC6-specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16167-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16167-1-AP (HDAC6-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 16167-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16167-1-AP (HDAC6-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 16167-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16167-1-AP (HDAC6-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 16167-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16167-1-AP (HDAC6-specific antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 16167-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16167-1-AP (HDAC6-specific antibody at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, HT-1080.Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

16167-1-AP bindet in WB, IHC, ELISA HDAC6-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone deacetylase 6
• Berechnetes Molekulargewicht: 131 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC005872
• Gene symbol: HDAC6
• Gene ID (NCBI): 10013
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC6 is a member of the class II mammalian histone deacetylases. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. This antibody is specific to HDAC6. It does not cross react with other HDACs.

Publikationen

Species	Application	Title
mouse	WB,IHC	Oncogene; MicroRNA-22 represses glioma development via activation of macrophage-mediated innate and adaptive immune responses.; Authors - Jiajie Tu
rat	WB	J Cell Mol Med; HDAC4/5-HMGB1 signalling mediated by NADPH oxidase activity contributes to cerebral ischaemia/reperfusion injury.; Authors - He Min M