HDAC8 Polyklonaler Antikörper

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• KD/KO Validated

HDAC8 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(32)

Kat-Nr. : 17548-1-AP

Synonyme

Protein decrotonylase HDAC8, Protein deacetylase HDAC8, HDAC 8, EC:3.5.1.98, EC:3.5.1.-

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  WB analysis using 17548-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17548-1-AP (HDAC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 17548-1-AP

  IP result of anti-HDAC8 (IP:17548-1-AP, 4ug; Detection:17548-1-AP 1:600) with Raji cells lysate 2800ug.

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  IHC staining of human lung cancer using 17548-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17548-1-AP (HDAC8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 17548-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17548-1-AP (HDAC8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 17548-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17548-1-AP (HDAC8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Raji-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 32 publications below
• IHC: See 2 publications below

Produktinformation

17548-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA HDAC8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HDAC8 fusion protein Ag11696
• Vollständiger Name: histone deacetylase 8
• Berechnetes Molekulargewicht: 377 aa, 42 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC050433
• Gene symbol: HDAC8
• Gene ID (NCBI): 55869
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone deacetylases (HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 8 was primarily found in the nucleus. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. This antibody is a rabbit polyclonal antibody raised against full length HDAC8 of human origin.

Publikationen

Species	Application	Title
human	WB	Cell; Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.; Authors - Xun Huang
rat	WB	Cell Rep; Gut microbiota shapes social dominance through modulating HDAC2 in the medial prefrontal cortex.; Authors - Tian Wang
mouse, rat	WB	Kidney Int; Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.; Authors - Xiaojie Wang
human	WB	Oncotarget; Thrombospondin-1 might be a therapeutic target to suppress RB cells by regulating the DNA double-strand breaks repair.; Authors - Pei Chen
human	WB	Oncogenesis; USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3.; Authors - Wei Zhan
human	WB	Molecules; Anti-Colorectal Cancer Activity of Solasonin from Solanum nigrum L. via Histone Deacetylases-Mediated p53 Acetylation Pathway; Authors - Xintian Lan