HDDC2 Monoklonaler Antikörper

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HDDC2 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E1B2

Kat-Nr. : 68391-1-PBS

Synonyme

EC:3.1.3.89, CGI-130, CGI 130, C6orf74, 5'-deoxynucleotidase HDDC2

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  WB analysis using 68391-1-Ig (same clone as 68391-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68391-1-Ig (HDDC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68391-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68391-1-Ig (same clone as 68391-1-PBS)

  WB result of HDDC2 antibody (68391-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDDC2 transfected HeLa cells. This data was developed using the same antibody clone with 68391-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 68391-1-Ig (same clone as 68391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68391-1-Ig (HDDC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68391-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 68391-1-Ig (same clone as 68391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68391-1-Ig (HDDC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68391-1-PBS in a different storage buffer formulation.

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  IF Staining of Saos-2 using 68391-1-Ig (same clone as 68391-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using HDDC2 antibody (68391-1-Ig, Clone: 2E1B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68391-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 68391-1-Ig (same clone as 68391-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.4 ug HDDC2 Monoclonal antibody (68391-1-Ig, Clone:2E1B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68391-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68391-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA HDDC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HDDC2 fusion protein Ag33258
• Vollständiger Name: HD domain containing 2
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC003357
• Gene symbol: HDDC2
• Gene ID (NCBI): 51020
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HDDC2, also named as C6orf74, plays a role in the maintenance of pluripotency in human ES and iPS cells. HDDC2 is a nuclear‐encoded but mitochondrially localized protein. It has been reported as associated with oxidative metabolism.