HDGF Monoklonaler Antikörper

HDGF Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5A7G3

Kat-Nr. : 60064-1-PBS

Synonyme

5A7G3, Hepatoma-derived growth factor, High mobility group protein 1-like 2, HMG-1L2

---

• 

  WB analysis of SMMC-7721 using 60064-1-Ig (same clone as 60064-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 60064-1-Ig (same clone as 60064-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 60064-1-Ig (same clone as 60064-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 60064-1-Ig (same clone as 60064-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 60064-1-Ig (same clone as 60064-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 60064-1-Ig (same clone as 60064-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

60064-1-PBS bindet in WB, IHC, Indirect ELISA HDGF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HDGF fusion protein Ag1893
• Vollständiger Name: hepatoma-derived growth factor (high-mobility group protein 1-like)
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC018991
• Gene symbol: HDGF
• Gene ID (NCBI): 3068
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HDGF, also named as HMG1L2, belongs to the HDGF family. It is a heparin-binding protein, with mitogenic activity for fibroblasts. HDGF acts as a transcriptional repressor. It plays important roles in the development and progression of cancers. HDGF may have a synergistic effect with VEGF during cancer development and Progression. (PMID:20848225)