HDGF2 Polyklonaler Antikörper

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• KD/KO Validated

HDGF2 Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

IHC, IF/ICC, CoIP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Rezensionen (1)

Kat-Nr. : 15134-1-AP

Synonyme

HDGFRP2, HRP 2, Hepatoma-derived growth factor 2, HDGF-related protein 2, HDGFL2

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  IHC staining of human colon using 15134-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15134-1-AP (HRP 2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 15134-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15134-1-AP (HRP 2 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 15134-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using HDGF2 antibody (15134-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 15134-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDGF2 antibody (15134-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 15134-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15134-1-AP (HRP 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, A431-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

15134-1-AP bindet in IHC, IF/ICC, CoIP, chIP, ELISA HDGF2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HDGF2 fusion protein Ag7281
• Vollständiger Name: hepatoma-derived growth factor-related protein 2
• Berechnetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC000755
• Gene symbol: HDGF2
• Gene ID (NCBI): 84717
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HDGF2, also known as HDGFL2 (Hepatoma-Derived Growth Factor-like 2), is a gene that encodes a member of the hepatoma-derived growth factor (HDGF) family. HDGF2 plays a role in LEDGF/p75-independent HIV-1 replication by determining HIV-1 integration site selection. It is also involved in the binding to methylated histones, which suggests a role in epigenetic regulation.

Publikationen

Species	Application	Title
human	WB,IF	Sci Transl Med; Mis-spliced transcripts generate de novo proteins in TDP-43-related ALS/FTD; Authors - Sahba Seddighi
human	WB	Sci Adv; The H3K36me2 writer-reader dependency in H3K27M-DIPG.; Authors - Jia-Ray Yu ; KD Validated
human	WB	Sci Adv; LEDGF and HDGF2 relieve the nucleosome-induced barrier to transcription in differentiated cells.; Authors - Gary LeRoy
mouse	WB,chIP	Nucleic Acids Res; HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription.; Authors - Xu Zhu ; KD Validated
human	IF, IP	Nucleic Acids Res; Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination.; Authors - Annika Baude
human		Mov Disord; TDP-43 Cryptic RNAs in Perry Syndrome: Differences across Brain Regions and TDP-43 Proteinopathies; Authors - Sarah R Pickles

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (12-04-2023)

Works in ICC (nuclear staining) and WB (no non specific bands)

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human / SH-SY5Y cells

HDGF2 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Human / SH-SY5Y cells (Cat no:15134-1-AP)