HDGFRP3 Polyklonaler Antikörper

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• KD/KO Validated

HDGFRP3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 12380-1-AP

Synonyme

CGI 142, HDGF 2, HDGF2, HDGFRP3, HRP 3, HRP-3

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Galerie der Validierungsdaten

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  WB analysis using 12380-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12380-1-AP (HDGFRP3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12380-1-AP

  IP Result of anti-HDGFRP3 (IP:12380-1-AP, 3ug; Detection:12380-1-AP 1:300) with mouse testis tissue lysate 4800ug.

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  IHC staining of human prostate cancer using 12380-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12380-1-AP (HDGFRP3 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of MCF-7 using 12380-1-AP

  Immunofluorescent analysis of MCF-7 cells, using HDGFRP3 antibody 12380-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

12380-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA HDGFRP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HDGFRP3 fusion protein Ag3046
• Vollständiger Name: hepatoma-derived growth factor, related protein 3
• Berechnetes Molekulargewicht: 203 aa, 23 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC015483
• Gene symbol: HDGFRP3
• Gene ID (NCBI): 50810
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HDGFRP3, also named as Hepatoma-derived growth factor-related protein 3 or HDGF2, is a 203 amino acid protein, which contains 1 PWWP domain and belongs to the HDGF family. HDGFRP3 detected in testis, heart, spinal cord and brain. HDGFRP3 as a nuclear potein enhances DNA synthesis and may play a role in cell proliferation. HDGFRP3 is a 30 kDa protein for phosphor modification.

Publikationen

Species	Application	Title
human	WB,IP,IF	Nucleic Acids Res; HDGFRP3 interaction with 53BP1 promotes DNA double-strand break repair; Authors - Zhen Zhang
human	IHC	Front Immunol; A robust CD8+ T cell-related classifier for predicting the prognosis and efficacy of immunotherapy in stage III lung adenocarcinoma; Authors - Jinteng Feng
human	WB,IF	Biochem Biophys Res Commun; Depletion of hepatoma-derived growth factor-related protein-3 induces apoptotic sensitization of radioresistant A549 cells via reactive oxygen species-dependent p53 activation.; Authors - Yun Hong Shik HS ; KD Validated
human	WB	Biochem Biophys Res Commun; Knockdown of hepatoma-derived growth factor-related protein-3 induces apoptosis of H1299 cells via ROS-dependent and p53-independent NF-κB activation.; Authors - Hong Shik Yun
human	WB,IHC,IF,ChIP	Cancer Sci; A novel function of HRP-3 in regulating cell cycle progression via the HDAC-E2F1-Cyclin E pathway in lung cancer; Authors - Hong Shik Yun ; KD Validated

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