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HDGFRP3 Polyklonaler Antikörper

HDGFRP3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12380-1-PBS

Synonyme

HDGF-2, HDGF2, HDGF 2, CGI-142, CGI142

Filter:
  • Western Blot (WB) analysis of various lysates using HDGFRP3 Polyclonal antibody (12380-1-AP)

    WB analysis using 12380-1-AP (same clone as 12380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12380-1-AP (HDGFRP3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12380-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using HDGFRP3 Polyclonal antibody (12380-1-AP)

    IP experiment of mouse testis using 12380-1-AP (same clone as 12380-1-PBS)

    IP result of anti-HDGFRP3 (IP:12380-1-AP, 3ug; Detection:12380-1-AP 1:300) with mouse testis tissue lysate 4800ug. This data was developed using the same antibody clone with 12380-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HDGFRP3 Polyclonal antibody (12380-1-AP)

    IHC staining of human prostate cancer using 12380-1-AP (same clone as 12380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12380-1-AP (HDGFRP3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12380-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using HDGFRP3 Polyclonal antibody (12380-1-AP)

    IF Staining of MCF-7 using 12380-1-AP (same clone as 12380-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HDGFRP3 antibody (12380-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12380-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

12380-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA HDGFRP3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HDGFRP3 fusion protein Ag3046
Vollständiger Name hepatoma-derived growth factor, related protein 3
Berechnetes Molekulargewicht 203 aa, 23 kDa
Beobachtetes Molekulargewicht 30 kDa
GenBank-ZugangsnummerBC015483
Gene symbol HDGFRP3
Gene ID (NCBI) 50810
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HDGFRP3, also named as Hepatoma-derived growth factor-related protein 3 or HDGF2, is a 203 amino acid protein, which contains 1 PWWP domain and belongs to the HDGF family. HDGFRP3 detected in testis, heart, spinal cord and brain. HDGFRP3 as a nuclear potein enhances DNA synthesis and may play a role in cell proliferation. HDGFRP3 is a 30 kDa protein for phosphor modification.