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HE4 Monoklonaler Antikörper

HE4 Monoklonal Antikörper für IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5B2D9

Kat-Nr. : 66557-1-PBS

Synonyme

WFDC2, 5B2D9, Epididymal secretory protein E4, Major epididymis-specific protein E4, WAP four disulfide core domain protein 2

Filter:
  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using HE4 Monoclonal antibody (66557-1-Ig)

    IHC staining of human ovary tumor using 66557-1-Ig (same clone as 66557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66557-1-Ig (HE4 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66557-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using HE4 Monoclonal antibody (66557-1-Ig)

    IHC staining of human ovary tumor using 66557-1-Ig (same clone as 66557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66557-1-Ig (HE4 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66557-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using HE4 Monoclonal antibody (66557-1-Ig)

    IF Staining of SKOV-3 using 66557-1-Ig (same clone as 66557-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using HE4 antibody (66557-1-Ig, Clone: 5B2D9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66557-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51083-2

    Cytometric bead array standard curve of MP51083-2

    Cytometric bead array standard curve of MP51083-2, HE4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66557-1-PBS. Detection antibody: 66557-3-PBS. Standard:Ag5936. Range: 0.781-100 ng/mL.

Geprüfte Anwendungen

Produktinformation

66557-1-PBS bindet in IHC, IF/ICC, Cytometric bead array, Indirect ELISA HE4 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen HE4 fusion protein Ag5936
Vollständiger Name WAP four-disulfide core domain 2
Berechnetes Molekulargewicht 13 kDa
GenBank-ZugangsnummerBC046106
Gene symbol HE4
Gene ID (NCBI) 10406
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WFDC2, also named as HE4, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is expressed in a number of normal tissues, and it is also highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. In some study, HE4 was referred to as a biomarker for ovarian carcinoma.