HECTD1 Polyklonaler Antikörper

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• KD/KO Validated

HECTD1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20605-1-PBS

Synonyme

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  WB analysis using 20605-1-AP (same clone as 20605-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20605-1-AP (HECTD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20605-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 20605-1-AP (same clone as 20605-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 20605-1-AP (HECTD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20605-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 20605-1-AP (same clone as 20605-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 20605-1-AP (HECTD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20605-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20605-1-AP (same clone as 20605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20605-1-AP (HECTD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20605-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20605-1-AP (same clone as 20605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20605-1-AP (HECTD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20605-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20605-1-AP (same clone as 20605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 20605-1-AP (HECTD1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20605-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20605-1-AP (same clone as 20605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 20605-1-AP (HECTD1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20605-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 20605-1-AP (same clone as 20605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 20605-1-AP (HECTD1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20605-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 20605-1-AP (same clone as 20605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 20605-1-AP (HECTD1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20605-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 20605-1-AP (same clone as 20605-1-PBS)

  Immunofluorescent analysis of Hela cells, using HECTD1 antibody 20605-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20605-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20605-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HECTD1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HECTD1 fusion protein Ag14581
• Vollständiger Name: HECT domain containing 1
• Berechnetes Molekulargewicht: 2612 aa, 290 kDa
• Beobachtetes Molekulargewicht: 290 kDa
• GenBank-Zugangsnummer: BC006237
• Gene symbol: HECTD1
• Gene ID (NCBI): 25831
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HECTD1 (E3 ubiquitin-protein ligase), which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates,may be required for development of the head mesenchyme and neural tube closure.It is also involved in sporadic Parkinson's disease(PMID:16714300).This protein is specific to HECTD1.