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HECW2 Polyklonaler Antikörper

HECW2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32026-1-AP

Synonyme

NEDD4-like E3 ubiquitin-protein ligase 2, KIAA1301, HECT-type E3 ubiquitin transferase HECW2, HECT, C2 and WW domain-containing protein 2, EC:2.3.2.26

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  • Western Blot (WB) analysis of various lysates using HECW2 Polyclonal antibody (32026-1-AP)

    WB analysis using 32026-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32026-1-AP (HECW2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using HECW2 Polyclonal antibody (32026-1-AP)

    IHC staining of mouse cerebellum using 32026-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32026-1-AP (HECW2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using HECW2 Polyclonal antibody (32026-1-AP)

    IHC staining of rat cerebellum using 32026-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 32026-1-AP (HECW2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using HECW2 Polyclonal antibody (32026-1-AP)

    IF Staining of A549 using 32026-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HECW2 antibody (32026-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMDA-MB-468-Zellen, U-118 MG cells
Erfolgreiche Detektion in IHCMaus-Cerebellum-Gewebe, Ratten-Cerebellum-Gewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCA549-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

32026-1-AP bindet in WB, IHC, IF/ICC, ELISA HECW2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HECW2 fusion protein Ag36316
Vollständiger Name HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
Berechnetes Molekulargewicht176 kDa，1572aa
Beobachtetes Molekulargewicht240 kDa
GenBank-ZugangsnummerXM_017004563.1
Gene symbol HECW2
Gene ID (NCBI) 57520
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HECW2, also known as NEDL2 (Nedd4-like E3 ubiquitin-protein ligase 2), is a member of the HECT (Homologous to the E6-AP Carboxyl Terminus) family of E3 ubiquitin ligases. It is crucial in various pathophysiological processes, including cell proliferation, apoptosis, autophagy, immune response, and DNA damage repair (PMID: 39529070). HECW2 is known to stabilize and enhance the transcriptional activity of p73, a member of the p53 family of tumor suppressors, which promotes cell cycle arrest and apoptosis and has an essential role in neurodevelopment (PMID: 27389779). In cancer, HECW2 is a promising target for tumor therapy due to its role in regulating cell cycle progression and angiogenesis (PMID: 39529070). In neurodevelopmental disorders, variants in HECW2 have been reported to cause a disorder characterized by hypotonia, seizures, and impaired language (PMID: 34321324).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for HECW2 antibody 32026-1-APProtokoll herunterladen
IHC protocol for HECW2 antibody 32026-1-APProtokoll herunterladenl
IF protocol for HECW2 antibody 32026-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Nikolett (Verified Customer) (09-25-2025)

This antibody is working on human lung cancer cell lines for Western Blotting after overnight incubation. The band was a bit faint but after exposure improvements I achieved a nice image. I recommend this antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human Lung Cancer Cell Lines