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HELIOS Monoklonaler Antikörper

HELIOS Monoklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F1F10

Kat-Nr. : 68558-1-PBS

Synonyme

HELIOS, IKZF2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2

Filter:
  • Western Blot (WB) analysis of Jurkat cells using HELIOS Monoclonal antibody (68558-1-Ig)

    WB analysis of Jurkat using 68558-1-Ig (same clone as 68558-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68558-1-Ig (HELIOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68558-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MOLT-4 cells using HELIOS Monoclonal antibody (68558-1-Ig)

    WB analysis of MOLT-4 using 68558-1-Ig (same clone as 68558-1-PBS)

    MOLT-4 cells were subjected to SDS PAGE followed by western blot with 68558-1-Ig (IKZF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68558-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using HELIOS Monoclonal antibody (68558-1-Ig)

    IP experiment of Jurkat using 68558-1-Ig (same clone as 68558-1-PBS)

    IP result of anti-HELIOS (IP:68558-1-Ig, 4ug; Detection:68558-1-Ig 1:3000) with Jurkat cells lysate 1560 ug. This data was developed using the same antibody clone with 68558-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68558-1-PBS bindet in WB, IP, Indirect ELISA HELIOS und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen HELIOS fusion protein Ag4357
Vollständiger Name IKAROS family zinc finger 2 (Helios)
Berechnetes Molekulargewicht 500 aa, 57 kDa
Beobachtetes Molekulargewicht 65 kDa
GenBank-ZugangsnummerBC028936
Gene symbol HELIOS
Gene ID (NCBI) 22807
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IKZF2 (Helios), a member of the Ikaros transcription factor family, is expressed in a majority of Treg cells and some T cell and lymphocyte subsets. The expression of IKZF2 is associated with T cell activation and cellular division and is suggested a suitable marker to distinguish natural and induced Treg (PMID:21918685). IKZF2 plays a crucial role in maintaining function and stability of Treg cells, and IKZF2 deficiency reduces tumor growth in mice (PMID: 36863346).