HEMGN Polyklonaler Antikörper

HEMGN Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 17616-1-AP

Synonyme

EDAG, EDAG 1, HEMGN, hemogen, Hemopoietic gene protein, NDR, PRO1620

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  WB analysis of Jurkat using 17616-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17616-1-AP (HEMGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 17616-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17616-1-AP (HEMGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 17616-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17616-1-AP (HEMGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 17616-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17616-1-AP (HEMGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 17616-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17616-1-AP (HEMGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 17616-1-AP

  IP result of anti-HEMGN (IP:17616-1-AP, 4ug; Detection:17616-1-AP 1:500) with HL-60 cells lysate 3000ug.

• 

  IHC staining of human testis using 17616-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17616-1-AP (HEMGN antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17616-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17616-1-AP (HEMGN antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HL-60-Zellen, humanes Hodengewebe, Maushodengewebe
• Erfolgreiche IP: HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

17616-1-AP bindet in WB, IP, IHC, ELISA HEMGN und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HEMGN fusion protein Ag11793
• Vollständiger Name: hemogen
• Berechnetes Molekulargewicht: 483 aa, 55 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC048324
• Gene symbol: HEMGN
• Gene ID (NCBI): 55363
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Biomedicines; Establishing Molecular Subgroups of CD8+ T Cell-Associated Genes in the Ovarian Cancer Tumour Microenvironment and Predicting the Immunotherapy Response; Authors - Yunshu Zhu