HEPACAM Polyklonaler Antikörper

HEPACAM Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18177-1-PBS

Synonyme

glialCAM, Hepatic and glial cell adhesion molecule, Protein hepaCAM

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  WB analysis of mouse brain using 18177-1-AP (same clone as 18177-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18177-1-AP (HEPACAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18177-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 18177-1-AP (same clone as 18177-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 18177-1-AP (HEPACAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18177-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 18177-1-AP (same clone as 18177-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18177-1-AP (HEPACAM antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18177-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 18177-1-AP (same clone as 18177-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18177-1-AP (HEPACAM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18177-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 18177-1-AP (same clone as 18177-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18177-1-AP (HEPACAM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18177-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 18177-1-AP (same clone as 18177-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18177-1-AP (HEPACAM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18177-1-PBS in a different storage buffer formulation.

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  IP experiment of rat brain using 18177-1-AP (same clone as 18177-1-PBS)

  IP result of anti-HEPACAM (IP:18177-1-AP, 4ug; Detection:18177-1-AP 1:500) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 18177-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 18177-1-AP (same clone as 18177-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18177-1-AP (HEPACAM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18177-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 18177-1-AP (same clone as 18177-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18177-1-AP (HEPACAM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18177-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 18177-1-AP (same clone as 18177-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18177-1-AP (HEPACAM Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18177-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 18177-1-AP (same clone as 18177-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18177-1-AP (HEPACAM Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18177-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 18177-1-AP (same clone as 18177-1-PBS)

  mmunofluorescent analysis of MCF-7 cells, using HEPACAM antibody 18177-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 18177-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18177-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA HEPACAM und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HEPACAM fusion protein Ag12870
• Vollständiger Name: hepatocyte cell adhesion molecule
• Berechnetes Molekulargewicht: 416 aa, 46 kDa
• Beobachtetes Molekulargewicht: 46-72 kDa
• GenBank-Zugangsnummer: BC104831
• Gene symbol: HEPACAM
• Gene ID (NCBI): 220296
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HepaCAM (hepatocyte cell adhesion molecule), also known as GlialCAM, is a single-pass type I membrane glycoprotein of 416 amino acids. It displays a typical structure of immunoglobulin (Ig)-like adhesion molecules including two extracellular Ig-like domains, a transmembrane segment, and a cytoplasmic tail. It has been shown that hepaCAM forms a cis-homodimer on the cell surface, and modulates cell-matrix interaction. It is predominantly expressed in the CNS glial cells. Defects in HEPACAM gene are the cause of megalencephalic leukoencephalopathy with subcortical cysts type 2A (MLC2A). HEPACAM has also been suggested as a tumor suppressor gene.