HER2/ErbB2 Rekombinanter Antikörper

HER2/ErbB2 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241366E6

Kat-Nr. : 98153-1-RR

Synonyme

Her2 / ErbB2, Her2, Erbb2, EC:2.7.10.1, CD340

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  FC experiment of NIH/3T3 using 98153-1-RR

  1x10^6 NIH/3T3 cells were surface stained with 0.25 ug Anti-Mouse HER2/ErbB2 Rabbit Recombinant Antibody (98153-1-RR, Clone: 241366E6) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98153-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98153-1-RR against Mouse HER2/ErbB2 were performed. The affinity constant is 1.50 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: NIH/3T3-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98153-1-RR bindet in FC HER2/ErbB2 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• Berechnetes Molekulargewicht: 139 kDa
• GenBank-Zugangsnummer: NM_001003817.1
• Gene symbol: Erbb2
• Gene ID (NCBI): 13866
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

HER2, also known as ErbB2, Neu, and CD340, is a 185-kDa transmembrane glycoprotein member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.