HER2/ErbB2 Rekombinanter Antikörper

HER2/ErbB2 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242436C4

Kat-Nr. : 98304-3-RR

Synonyme

ERBB2, CD340, HER 2, HER 2/neu, HER2

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  FC experiment of MCF-7 using 98304-3-RR

  1x10^6 MCF-7 cells were surface stained with 0.25 ug Anti-Human HER2/ErbB2 Rabbit RecAb (98304-3-RR, Clone: 242436C4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of Transfected CHO using 98304-3-RR

  1x10^6 CHO cells or HER2/ErbB2-transfected CHO cells were surface stained with 0.25 ug Anti-Human HER2/ErbB2 Rabbit RecAb (98304-3-RR, Clone: 242436C4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of Transfected CHO using 98304-3-RR

  1x10^6 CHO cells or HER2/ErbB2-transfected CHO cells were surface stained with 0.25 ug Anti-Human HER2/ErbB2 Rabbit RecAb (98304-3-RR, Clone: 242436C4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98304-3-RR

  Biolayer interferometry (BLl) kinetic assays of 98304-3-RR against Human HER2/ErbB2 were performed. The affinity constant is 3.53 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: MCF-7-Zellen, Transfected CHO cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98304-3-RR bindet in FC HER2/ErbB2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• Berechnetes Molekulargewicht: 1255 aa, 138 kDa
• GenBank-Zugangsnummer: BC156755
• Gene symbol: HER2/ErbB2
• Gene ID (NCBI): 2064
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

HER2, also known as ErbB2, Neu, and CD340, is a 185-kDa transmembrane glycoprotein member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.