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HERC2 Polyklonaler Antikörper

HERC2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27459-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using HERC2 Polyclonal antibody (27459-1-AP)

    WB analysis using 27459-1-AP (same clone as 27459-1-PBS)

    HeLa cell lysates were subjected to SDS PAGE followed by western blot with 27459-1-AP (HERC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27459-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HERC2 Polyclonal antibody (27459-1-AP)

    IHC staining of human breast cancer using 27459-1-AP (same clone as 27459-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27459-1-AP (HERC2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27459-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HERC2 Polyclonal antibody (27459-1-AP)

    IHC staining of human liver cancer using 27459-1-AP (same clone as 27459-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27459-1-AP (HERC2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27459-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using HERC2 Polyclonal antibody (27459-1-AP)

    IF Staining of U2OS using 27459-1-AP (same clone as 27459-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HERC2 antibody (27459-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27459-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

27459-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HERC2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HERC2 fusion protein Ag26501
Vollständiger Name hect domain and RLD 2
Berechnetes Molekulargewicht527 kDa
Beobachtetes Molekulargewicht527 kDa
GenBank-ZugangsnummerBC047911
Gene symbol HERC2
Gene ID (NCBI) 8924
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.