HERC4 Polyklonaler Antikörper

HERC4 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 13691-1-AP

Synonyme

Probable E3 ubiquitin-protein ligase HERC4, KIAA1593, HECT-type E3 ubiquitin transferase HERC4, hect domain and RLD 4, HECT domain and RCC1-like domain-containing protein 4

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  WB analysis of human brain using 13691-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13691-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13691-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13691-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13691-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 13691-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13691-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13691-1-AP

  IP result of anti-HERC4 (IP:13691-1-AP, 4ug; Detection:13691-1-AP 1:800) with HeLa cells lysate 2000ug.

• 

  IF Staining of HeLa using 13691-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HERC4 antibody (13691-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, humanes Plazenta-Gewebe, humanes Hodengewebe, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

13691-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA HERC4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HERC4 fusion protein Ag4566
• Vollständiger Name: hect domain and RLD 4
• Berechnetes Molekulargewicht: 1049 aa, 118 kDa
• Beobachtetes Molekulargewicht: 107 kDa
• GenBank-Zugangsnummer: BC039600
• Gene symbol: HERC4
• Gene ID (NCBI): 26091
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HERC4, also known as HECT and RLD domain containing E3 ubiquitin protein ligase 4, is a protein encoded by the HERC4 gene in humans. HERC4 is a novel member of HERC family. The mRNA of HERC4 has been found in all examined tissues, with its levels being significantly higher in the brain and testis than in the placenta and heart. (PMID: 28430527)

Publikationen

Species	Application	Title
human	WB,IHC,IF,IP	Elife; SOX4 facilitates PGR protein stability and FOXO1 expression conducive for human endometrial decidualization.; Authors - Pinxiu Huang
human	WB,CoIP	Cancer Biother Radiopharm; METTL3 and HERC4: Elevated Expression and Impact on Hepatocellular Carcinoma Progression; Authors - Tao Sun

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chun (Verified Customer) (07-03-2019)

This antibody is excellent.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2,000
• Cell Tissue Type: HEK293 and HaCaT cells