HERC4 Polyklonaler Antikörper

HERC4 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13691-1-PBS

Synonyme

Probable E3 ubiquitin-protein ligase HERC4, KIAA1593, HECT-type E3 ubiquitin transferase HERC4, hect domain and RLD 4, HECT domain and RCC1-like domain-containing protein 4

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  WB analysis of human brain using 13691-1-AP (same clone as 13691-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

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  WB analysis using 13691-1-AP (same clone as 13691-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13691-1-AP (same clone as 13691-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13691-1-AP (same clone as 13691-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 13691-1-AP (same clone as 13691-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 13691-1-AP (same clone as 13691-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 13691-1-AP (same clone as 13691-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 13691-1-AP (same clone as 13691-1-PBS)

  IP result of anti-HERC4 (IP:13691-1-AP, 4ug; Detection:13691-1-AP 1:800) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13691-1-AP (same clone as 13691-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HERC4 antibody (13691-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13691-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA HERC4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HERC4 fusion protein Ag4566
• Vollständiger Name: hect domain and RLD 4
• Berechnetes Molekulargewicht: 1049 aa, 118 kDa
• Beobachtetes Molekulargewicht: 107 kDa
• GenBank-Zugangsnummer: BC039600
• Gene symbol: HERC4
• Gene ID (NCBI): 26091
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HERC4, also known as HECT and RLD domain containing E3 ubiquitin protein ligase 4, is a protein encoded by the HERC4 gene in humans. HERC4 is a novel member of HERC family. The mRNA of HERC4 has been found in all examined tissues, with its levels being significantly higher in the brain and testis than in the placenta and heart. (PMID: 28430527)