HERC4 Rekombinanter Antikörper

HERC4 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242599B7

Kat-Nr. : 84952-5-PBS

Synonyme

242599B7, EC:2.3.2.26, HECT domain and RCC1-like domain-containing protein 4, hect domain and RLD 4, HECT-type E3 ubiquitin transferase HERC4

---

• 

  WB analysis using 84952-5-RR (same clone as 84952-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84952-5-RR (HERC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84952-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 84952-5-RR (same clone as 84952-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84952-5-RR (HERC4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84952-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84952-5-RR (same clone as 84952-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HERC4 antibody (84952-5-RR, Clone: 242599B7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84952-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84952-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84952-5-RR against Human HERC4 were performed. The affinity constant is 0.51 nM.

---

Geprüfte Anwendungen

Produktinformation

84952-5-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HERC4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HERC4 fusion protein Ag4566
• Vollständiger Name: hect domain and RLD 4
• Berechnetes Molekulargewicht: 1049 aa, 118 kDa
• Beobachtetes Molekulargewicht: 107 kDa
• GenBank-Zugangsnummer: BC039600
• Gene symbol: HERC4
• Gene ID (NCBI): 26091
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HERC4, also known as HECT and RLD domain containing E3 ubiquitin protein ligase 4, is a protein encoded by the HERC4 gene in humans. HERC4 is a novel member of HERC family. The mRNA of HERC4 has been found in all examined tissues, with its levels being significantly higher in the brain and testis than in the placenta and heart. (PMID: 28430527)