HERPUD1 Rekombinanter Antikörper

HERPUD1 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

243054E4

Kat-Nr. : 85627-1-PBS

Synonyme

HERP, Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein, KIAA0025, Methyl methanesulfonate, MIF1

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  WB analysis of BxPC-3 using 85627-1-RR (same clone as 85627-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 85627-1-RR (HERPUD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85627-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 85627-1-RR (same clone as 85627-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 85627-1-RR (HERPUD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85627-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 85627-1-RR (same clone as 85627-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 85627-1-RR (HERPUD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85627-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 85627-1-RR (same clone as 85627-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85627-1-RR (HERPUD1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85627-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 85627-1-RR (same clone as 85627-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85627-1-RR (HERPUD1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85627-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85627-1-PBS bindet in WB, IHC, Indirect ELISA HERPUD1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HERPUD1 fusion protein Ag34891
• Vollständiger Name: homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC008320
• Gene symbol: HERPUD1
• Gene ID (NCBI): 9709
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein (HERPUD1), also called HERP or MIF1, is component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. HERP is strongly induced by ER stress but rapidly degraded by proteasome. HERP interacts with members of the ubiquilin family, which function as a shuttle factor to deliver ubiquitinated substrates to the proteasome for degradation. HERP is present in activated microglia in senile plaques in the brain of patients with Alzheimer disease, and there is evidence suggesting HERP increase nigro-striatal dopaminergic activity and is able to improve symptoms in patients with Parkinson's disease, levodopa-related dyskinesias and Tardive dyskinesia. MW of HERP is 54 kDa (PMID: 23620059).