HERPUD2 Monoklonaler Antikörper

HERPUD2 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1H10H1

Kat-Nr. : 68427-1-Ig

Synonyme

FLJ22313, FLJ31032, HERPUD family member 2, HERPUD2

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  WB analysis using 68427-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68427-1-Ig (HERPUD2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IF Staining of HepG2 using 68427-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using HERPUD2 antibody (68427-1-Ig, Clone: 1H10H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NCI-H1299-Zellen, A549-Zellen, HepG2-Zellen, HT-29-Zellen, K-562-Zellen, LNCaP-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68427-1-Ig bindet in WB, IF/ICC, ELISA HERPUD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG3
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HERPUD2 fusion protein Ag15007
• Vollständiger Name: HERPUD family member 2
• Berechnetes Molekulargewicht: 406 aa, 45 kDa
• Beobachtetes Molekulargewicht: 35-45 kDa
• GenBank-Zugangsnummer: BC005091
• Gene symbol: HERPUD2
• Gene ID (NCBI): 64224
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HERPUD2, also named as Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 2 protein, is a 406 amino acid protein, which contains one ubiquitin-like domain. HERPUD2 as a single-pass membrane protein could be involved in the unfolded protein response (UPR) pathway. HERPUD2 may exstis as two isoforms 406aa,45 kDa and 297aa 33 kDa (Genebank:EAW94050).