HEXIM1 Rekombinanter Antikörper

HEXIM1 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242587A2

Kat-Nr. : 85028-1-PBS

Synonyme

242587A2, CLP 1, CLP1, EDG1, Estrogen down-regulated gene 1 protein

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  WB analysis using 85028-1-RR (same clone as 85028-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85028-1-RR (HEXIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85028-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85028-1-RR (same clone as 85028-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85028-1-RR (HEXIM1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85028-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85028-1-RR (same clone as 85028-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85028-1-RR (HEXIM1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85028-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85028-1-RR (same clone as 85028-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEXIM1 antibody (85028-1-RR, Clone: 242587A2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85028-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 85028-1-RR (same clone as 85028-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HEXIM1 Recombinant antibody (85028-1-RR, Clone:242587A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85028-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85028-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA HEXIM1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HEXIM1 fusion protein Ag8144
• Vollständiger Name: hexamethylene bis-acetamide inducible 1
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC006460
• Gene symbol: HEXIM1
• Gene ID (NCBI): 10614
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 65-70 kDa and 54 kDa ( PMID: 33627647, PMID: 20976203, PMID: 28254838).