HEXIM1 Polyklonaler Antikörper

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• KD/KO Validated

HEXIM1 Polyklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-15676

Synonyme

HEXIM 1, Hexamethylene bis-acetamide-inducible protein 1, Estrogen down-regulated gene 1 protein, EDG1, CLP1

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  IF Staining of HeLa using CL488-15676

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 HEXIM1 antibody (CL488-15676) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of HeLa using CL488-15676

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human HEXIM1 (CL488-15676) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-15676 bindet in IF/ICC, FC (Intra) HEXIM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HEXIM1 fusion protein Ag8144
• Vollständiger Name: hexamethylene bis-acetamide inducible 1
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC006460
• Gene symbol: HEXIM1
• Gene ID (NCBI): 10614
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 70 and 54 kDa. Catalog#15676-1-AP recognises both 70 and 54 kDa band.